How can aceto orcein stain chromatin?

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How can aceto orcein stain chromatin? – All helpful answers

• How does Aceto-orcein stain work?

  Aceto-orcein stain turns chromosomes a purple-red colour. This stain can be prepared from powder or purchased as solution; staining is best with freshly prepared stain as, over time, the stain precipitates and changes colour from a deep red or burgundy colour to a brownish colour.

• What structure does acetic orcein stain?

  Acetic orcein staining of polytene chromosomes was introduced in 1941 shortly after the initial studies on aceto-carmine-stained chromosomes by Bridges (2) and has remained a standard method of preparation.

• How does orcein stain work?

  With orcein staining, HBsAg antigens appear as irregular aggregates in the cytoplasm of host cells. Copper-related proteins are present in large quantities in cells during diseases such as Wilson’s disease or certain forms of cirrhosis. These proteins can be visualized through orcein stain.

• What is acetic orcein used for?

  Acetic-orcein is equally good for rapid chromosome counts. For root tips the addition of 1 cc. of N HC1 solution to 10 cc. of the standard solution together with gentle heating of the tissues in a drop of the mixture assists in the softening and separation of cells necessary for chromosome study

• What is the purpose of using Aceto orcein in mitosis experiment?

  Plant cells are glued together by a middle lamella of pectins. Hydrochloric acid will break down the pectins that hold the cell together. Acetic orcein will stain the chromosomes dark red and fix the cells, stopping mitosis.

• What stain is used to colour chromosomes?

  Acetocarmine and Giemsa stain can be used to stain the chromosomes.

• Why Acetocarmine stain is used in mitosis?

  Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the onion root tip cells can be visualized perfectly by treating with this stain.

• Which stain is used for staining chromatin?

  Abstract. Mixtures of polychrome methylene blue-eosin Y (i.e., Giemsa stain) are widely used in biological staining. They induce a striking purple coloration of chromatin DNA (the Romanowsky-Giemsa effect), which contrasts with the blue-stained RNA-containing cytoplasm and nucleoli.

• How do you make Aceto-Orcein stain?

  Aceto-orcein preparation (1% solution)

  This solution is prepared by pouring 55 mL boiling glacial acetic acid over 1 g orcein powder. The solution is cooled, 45 mL of distilled water added, and filtered. This solution is unstable and should be prepared fresh before use.

• Which dye is best suited for staining chromosomes?

  So, the correct option is ‘Carmine’.

• What is the mechanism of action of Acetocarmine?

  Acetocarmine is a non-specific nuclear stain which simply binds the chromosomes and gives colour to them. However, specific nuclear stain (e.g. feulgen) reacts with chromosomes to give them the colour. Acetocarmine is a dye obtained from insects.

Useful articles on How can aceto orcein stain chromatin?

ACETO-ORCEIN STAINING – Kansas State University

JARS v48n3 – An Improved Chromosome Staining Method …

Nuclear Sexing With Aceto-Orcein – jstor